Digestion with the enzyme is performed via a one-step procedure that is faster and simpler than other available methods. Unit Definition: One unit (1 U) is defined as the amount of enzyme required to degrade 1 µg of λ DNA in a total reaction volume of 25 µl for 1 hour at 37°C. Quick and simple procedure for completely degrading DNA into its individual nucleotide component for quantitative analysis (e.g., whole-genome methylation analysis by HPLC, TLC, etc.)1 hour, single-enzyme digest vs. Prolonged storage should be -70°C. Inactivation: Heat-inactivate at 70°C for 20 minutes.Shipping Weight: 0.125 lbsShipping Dimensions: 1.00 x 1.00 x 2.00Research or further manufacturing use only, not for food or drug use. conventional 6 - 16 hour multi-step enzyme digestion protocolsStorage Conditions: Freezer Format: Provided in solution (10 units/µl) w/ 10X Reaction Buffer Storage: Store at -20°C for up to 12 months. Reaction Conditions: DNA Degradase in 1X DNA Degradase Reaction Buffer. Avoid repeated freeze/thawing of reagents. DNA Degradase is ideal for whole-genome DNA methylation analysis by a number of downstream applications (i.e., HPLC, TLC, etc.). Incubate reaction mixtures at 37°C for 1 hour. DNA Degradase from Zymo Research is a nuclease mix that quickly and efficiently degrades DNA to its individual nucleotide components.
Lab Supplies & Consumables,Microbiology Media
DNA Degradase, 2000 Units
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